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ice cold phosphate buffered saline  (Servicebio Inc)

 
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    Servicebio Inc ice cold phosphate buffered saline
    Ice Cold Phosphate Buffered Saline, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ice cold phosphate buffered saline/product/Servicebio Inc
    Average 86 stars, based on 1 article reviews
    ice cold phosphate buffered saline - by Bioz Stars, 2026-06
    86/100 stars

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    Treatment procedure for ciliobrevin-D to the olfactory pits. 1. Fish were anesthetized <t>with</t> <t>ice-cold</t> water, and hold it upright position. 2. olfactory pits were indicated with arrows. 3. 40 µM ciliobrevin-D in 2 % agarose with 0.025% phenole red was air-injected into the posterior olfactory pit using a micropipette. 4.-5. After loading the ciliobrevin gel 6. Olfactory pits were covered by superglue 7. After curing (∼1 min), the fish were released in the fresh fish-conditioned water at ∼22°C, and their recovery was carefully observed until they actively swam. The superglue covers eventually fell off ∼30 min after curing.
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    Treatment procedure for ciliobrevin-D to the olfactory pits. 1. Fish were anesthetized with ice-cold water, and hold it upright position. 2. olfactory pits were indicated with arrows. 3. 40 µM ciliobrevin-D in 2 % agarose with 0.025% phenole red was air-injected into the posterior olfactory pit using a micropipette. 4.-5. After loading the ciliobrevin gel 6. Olfactory pits were covered by superglue 7. After curing (∼1 min), the fish were released in the fresh fish-conditioned water at ∼22°C, and their recovery was carefully observed until they actively swam. The superglue covers eventually fell off ∼30 min after curing.

    Journal: bioRxiv

    Article Title: Novel mechanisms of chemosensory adaptation to the cave environment

    doi: 10.64898/2026.04.03.716434

    Figure Lengend Snippet: Treatment procedure for ciliobrevin-D to the olfactory pits. 1. Fish were anesthetized with ice-cold water, and hold it upright position. 2. olfactory pits were indicated with arrows. 3. 40 µM ciliobrevin-D in 2 % agarose with 0.025% phenole red was air-injected into the posterior olfactory pit using a micropipette. 4.-5. After loading the ciliobrevin gel 6. Olfactory pits were covered by superglue 7. After curing (∼1 min), the fish were released in the fresh fish-conditioned water at ∼22°C, and their recovery was carefully observed until they actively swam. The superglue covers eventually fell off ∼30 min after curing.

    Article Snippet: The tissue around rosettes was carefully removed under the ice-cold RNase-free PBS (diethyl pyrocarbonate-treated PBS; ThermoFisher Scientific), washed once in the fresh ice-cold RNase-free PBS, recovered in the pre-weighed 1.5 mL tube (SP Bel-Art, Wayne, NJ, USA), and flash frozen by submerging it in liquid nitrogen.

    Techniques: Olfactory, Injection